Dr. Moshe Giladi

Physiology
פיזיולוגיה סגל אקדמי קליני
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Dr. Moshe Giladi
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Office: Sackler School of Medicine, 532

CV

2018-present       Senior Lecturer (Assistant Professor), Department of Physiology and Pharmacology, Sackler Faculty of Medicine, Tel Aviv University, Israel

2016                    Medical intern, Tel-Aviv Sourasky Medical Center, Tel-Aviv, Israel.

2014-2018           Research Associate, Sackler Faculty of Medicine, Tel Aviv University, Israel

​2014-2017          Junior teacher, Sacker Faculty of Medicine, Tel-Aviv University, Israel.

2012-2016          M.D., M.D/Ph.D. program, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel. Summa cum laude.

2014-2009        Ph.D., M.D./Ph.D. program, Department of Physiology and Pharmacology, Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel. Thesis title: “Molecular mechanisms underlying regulatory specificity of distinct variants in the Na+/Ca2+ exchanger superfamily”. Supervised by Prof. Daniel Khananshvili.

2006-2009           ​B.Med.Sc. (Medical Sciences), Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel. Cum laude.

Research

Over the past few decades, breakthroughs in genome sequencing have shed light on the genetic basis of numerous diseases. The identification of causative mutations is no longer rate limiting, but the molecular mechanisms leading to protein dysfunction and thereby to the clinical phenotypes often remain poorly understood. Thus, biochemical validation of the genetic data is essential to achieve a better understanding of disease mechanisms and the resulting therapeutic possibilities. My research focuses on elucidating the structural and functional basis of human diseases at the protein level using cutting-edge molecular methods from the fields of molecular biology, biochemistry and biophysics. 

 

Publications

  1. Giladi M., Lee S.Y., Refaeli B., Hiller R., Chung K.Y. & Khananshvili D. (2019) Structure-dynamic and functional relationships in a Li+-transporting sodium‑calcium exchanger mutant. BBA Bio, 1860, 189-200.
  2. Lisnyansky M., Kapelushnik N., Ben-Bassat A., Marom M., Loewenstein A., Khananshvili D., Giladi M.* & Haitin Y.* (2018) Bisphosphonates inhibition of GGPS1 mutant underlies Atypical Femoral Fractures. Mol Pharm, 94, 1-10.
  3. Van-Dijk L.*, Giladi M.*, Refaeli B., Hiller R., Hongying Cheng M., Bahar I., & Khananshvili D. (2018). Key residues controlling bidirectional ion movements in Na+/Ca2+ exchanger. Cell Calcium, 76, 10-22.
  4. Ferofontov A., Strulovich R., Marom M., Giladi M. & Haitin Y. (2018). Inherent flexibly of CLIC6 revealed by crystallographic and solution studies. Sci Rep. 8, 6882.
  5. Padala P., Soudah N., Giladi M., Haitin Y., Isupov M.N. & Wiener R. (2017). The crystal structure and conformations of an unbranched mixed tri-Ubiquitin chain containing K48 and K63 linkages. J Mol Biol. 429, 3801-3813
  6. Edri I., Goldenberg M., Lisnyansky M., Strulovich R., Newman H., Loewenstein A., Khananshvili D., Giladi M.* & Haitin Y*. (2017). Overexpression and purification of human cis-prenyltransferase in Escherichia coli. J Vis Exp. 3, 126
  7. Giladi M., van Dijk L, Refaeli B, Almagor L, Hiller R, Man P, Forest E, Khananshvili D. (2017). Dynamic distinctions in the sodium-calcium exchanger adopting the inward- and outward-facing conformational states. J Biol Chem. 292, 12311-12323
  8. Giladi M., Lee S.Y., Ariely Y., Teldan Y., Granit R., Strulovich R., Haitin Y., Chung K.Y. & Khananshvili D. (2017). Structure-based dynamic arrays in regulatory domains of sodium-calcium exchanger (NCX) isoforms. Sci Rep. 7, 993
  9. Giladi M., Edri I., Goldenberg M., Newman H., Strulovich R., Khananshvili D., Haitin Y., Loewenstein A. (2017). Purification and characterization of human dehydrodolychil diphosphate synthase (DHDDS) overexpressed in E. coli. Protein Expr Purif. 132, 138-142
  10. Giladi M., Shor R., Lisnyansky M. & Khananshvili D. (2016). Structure-functional basis of ion transport in sodium-calcium exchanger (NCX) proteins. Int J Mol Sci. 17, 1949
  11. Refaeli B, Giladi M., Hiller R. & Khananshvili D. (2016). Structure-based engineering of lithium transport capacity in an archaeal sodium-calcium exchanger (NCX_Mj). Biochemistry. 55, 1673-1676
  12. Giladi M., Tal I. & Khananshvili D. (2016). Structural features of ion transport and allosteric regulation in sodium-calcium exchanger (NCX) proteins. Front. Physiol. 7, 30
  13. Tal I., Kozlovsky T., Brisker D., Giladi M.* & Khananshvili D*. (2016) Kinetic and equilibrium properties of regulatory Ca2+-binding domains in sodium-calcium exchangers 2 and 3. Cell Calcium. 59, 181-188
  14. Lee S.Y.*, Giladi M.*, Bohbot H., Hiller R., Chung K.Y. & Khananshvili D. (2016). Structure-dynamic basis of splicing dependent regulation in tissue-specific variants of the sodium-calcium exchanger (NCX1). FASEB J. 30, 1356-1366
  15. Giladi M., Almagor L., van Dijk L., Hiller R., Man P., Forest E. & Khananshvili D. (2016). Asymmetric preorganization of inverted pair residues in the sodium-calcium exchanger. Sci Rep. 6, 20753
  16. Giladi M., Lee S.Y., Hiller R., Chung K.Y. & Khananshvili D. (2015). Structure-dynamic determinants governing a mode of regulatory response and propagation of allosteric signal in splice variants of Na+/Ca2+ exchange (NCX) proteins. Biochem J. 465, 489-501
  17. Marinelli F., Almagor L., Hiller R., Giladi M., Khananshvili D. & Faraldo-Gómez J.D. (2014). Sodium recognition by the Na+/Ca2+ exchanger in the outward-facing conformation. Proc Natl Acad Sci USA. 111, E5354-5362
  18. Almagor L., Giladi M., van Dijk L., Buki T., Hiller R. & Khananshvili D. (2014). Functional asymmetry of bidirectional Ca2+-movements in an archaeal sodium-calcium exchanger (NCX_Mj). Cell Calcium. 56, 276-284
  19. Giladi M., Michaely L., Almagor L., Bar-On D., Buki T., Ashery U., Khananshvili D. & Hirsch J.A. (2013). The C2B domain is the primary Ca2+ sensor in DOC2B: A structural and functional analysis. J Mol Biol. 425, 4629-4641
  20. Giladi M., Hiller R., Hirsch J.A. & Khananshvili D. (2013) Population shift underlies Ca2+-induced regulatory transitions in the sodium-calcium exchanger (NCX). J Biol Chem. 288, 23141-23149
  21. Giladi M. & Khananshvili D. (2013). Molecular determinants of allosteric regulation in NCX proteins. Adv Exp Med Biol. 961, 35-48
  22. Meisel E., Dvir M., Haitin Y., Giladi M., Peretz A. & Attali B. (2012). KCNQ1 channels do not undergo concerted but sequential gating transitions in both the absence and the presence of KCNE1 protein. J Biol Chem 287, 34212-34224
  23. Giladi M., Friedberg I., Fang X., Hiller R., Wang YX. & Khananshvili D. (2012). G503 is obligatory for coupling of regulatory domains in NCX proteins. Biochemistry. 51, 7313-7320
  24. Giladi M., Sasson Y., Fang X., Hiller R., Buki T., Wang Y-X., Hirsch J.A. & Khananshvili D. (2012). A common Ca2+-driven interdomain module governs eukaryotic NCX regulation. PLoS One. 7, e39985
  25. Giladi M., Bohbot H., Buki T., Schulze D.H., Hiller R. & Khananshvili D. (2012). Dynamic features of allosteric Ca2+ sensor in tissue-specific NCX variants. Cell Calcium. 51, 478-485
  26. Boyman L., Hagen B.M., Giladi M., Hiller R., Lederer W.J. & Khananshvili D. (2011). Proton-sensing Ca2+ binding domains regulate the cardiac Na+/Ca2+ exchanger. J Biol Chem. 286, 28811-28820
  27. Giladi M., Boyman L., Mikhasenko H., Hiller R. & Khananshvili D. (2010). Essential role of the CBD1-CBD2 linker in slow dissociation of Ca2+ from the regulatory two-domain tandem of NCX1. J Biol Chem. 285, 28117-28125

 

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