Optima XPN 80K
Ultracentrifuge can run at speeds as high as 80,000 rpm. Rotors spin in an evacuated and refrigerated chamber, to reduce friction and heating of rotor and samples.
Features:
|
Max Speed: |
80,000 rpm |
|
g Force: |
694,000 x g |
|
Set Speed: |
1,000 to 80,000 rpm in 100-rpm increments |
|
Set Temperature: |
0 to 40° C in 1° C increments |
Applications
- Separation of proteins by ammonium sulfate precipitation and sucrose/glycerol gradients
- Clarification of lysates and homogenates
- Fractionation of lipoproteins by differential floatation and density gradients
- Isolation of subcellular fractions (microsomes, mitochondria, nuclei, membranes, ribosomes) by pelleting
- Isolation of subcellular fractions (chromatin, mitochondria, ribosomes, microsomes, membranes) by sucrose gradients
- DNA size fractionation in gradients and plasmid purification in CsCl
- Pelleting in CsCl and CsTFA density gradients of RNA
- Isolation of viruses by pelleting and density gradients
Available rotors
Published procedures involving a centrifugation step usually cite the required speed in g-units (i.e. RCF or Relative Centrifugal Force). The centrifuge speed however, is set in rpm (Revolutions Per Minute). In order to convert g-values to rpm, for one of the rotors available in the lab, use the following link - http://www.sciencegateway.org/tools/rotor.htm, http://www.geneinfinity.org/sp/sp_rotor.html, or table:
|
Rotor |
max RCF |
Max speed |
Number of tubes X Nominal Capacity |
Angel description |
|
70.1 Ti |
450,000 |
70,000 |
12 x 13.5 mL |
Fixed angle 30o angle |
|
70 Ti |
504,000 |
70,000 |
8 X 39 mL |
Fixed angle 30o angle |
|
45 Ti |
235,000 |
45,000 |
6 X 94 mL |
Fixed angle 30o angle |
|
SW 55 Ti |
368,000 |
55,000 |
6 x 5 mL |
Swinging Bucket 90o angle |
|
SW 41 Ti |
288,000 |
41,000 |
6 x 13.2 mL |
Swinging Bucket 90o angle |
|
SW 28.1 |
150,000 |
28,000 |
6 x 17 mL |
Swinging Bucket 90o angle |
|
SW 28 |
141,000 |
28,000 |
6 x 38.5 mL |
Swinging Bucket 90o angle |
|
SW 32 Ti |
175000 |
32000 |
6 x 38.5 mL |
Swinging Bucket 90o angle |
|
SW 32.1 Ti |
187000 |
32000 |
6 x 17 mL |
Swinging Bucket 90o angle |
