Optima MAX-XP Benchtop Ultracentrifuges
The Beckman Optima™ MAX-XP is tabletop ultracentrifuge for small samples as 175 μL and at speeds of up to 150,000 RPM and more than 1,000,000 x g.
|
Maximum Speed |
150000 rpm |
|
Force |
543 000 G |
|
Temperature |
2 to 40 degrees C in 1 degree increments |
Applications
- Separation of proteins by ammonium sulfate precipitation and sucrose/glycerol gradients
- Clarification of lysates and homogenates
- Fractionation of lipoproteins by differential floatation and density gradients
- Isolation of subcellular fractions (microsomes, mitochondria, nuclei, membranes, ribosomes) by pelleting
- Isolation of subcellular fractions (chromatin, nucleosomes, microsomes, membranes) by sucrose gradients
- DNA size fractionation in gradients and plasmid purification in CsCl
- Pelleting in CsCl and CsTFA density gradients of RNA
Available rotors
Published procedures involving a centrifugation step usually cite the required speed in g-units (i.e. RCF or Relative Centrifugal Force). The centrifuge speed however, is set in rpm (Revolutions Per Minute). In order to convert g-values to rpm, for one of the rotors available in the lab, use the following link - http://www.sciencegateway.org/tools/rotor.htm, http://www.geneinfinity.org/sp/sp_rotor.html, or table:
|
Rotor |
max RCF |
Max speed |
Number of tubes X Nominal Capacity |
Angel description |
|
TLS-55 |
259000 |
55000 |
4 X 2.2 mL |
Swinging Bucket 90o angle |
|
TLA-100 |
436000 |
100000 |
20 X 0.2 mL |
Fixed angle 30o angle |
|
TLA-100.2 |
436000 |
100000 |
10 X 2 mL |
Fixed angle 30o angle |
|
MLS-50 |
268000 |
50000 |
4 X 5.0 mL |
Swinging Bucket 90o angle |
