Sapphire Biomolecular Imager

The Azure Sapphire Biomolecular Imager (488nmLD, 520nmLD, 658nmLD, 784nm LD) is a laser scanning system for the detection of fluorescence markers in gels, membranes and plates. The system supports long and short wavelengths of near infrared fluorescence (NIR), red/green/blue (RGB) imaging, chemiluminescent imaging, phosphor imaging as well as optical densitometry (OD).

Features

· Compatible with most radioisotopes including 3H, 11C, 14C, 18F, 32P, 33P, 35S, 99mTc, 125I

· Extremely sensitive: able to detect as little as 3pg of protein.

· Higher sensitivity and reduced exposure time compared to traditional film

· Maximum field of view: 25cm x 25cm, resolution 2688X2200.

· Post capture image processing (crop, rotation, contrast, and brightness adjustment) to enhance images for qualitative purposes

· Software to analyze and quantify images, includes automatic lane determination and band detection, background subtraction

Applications

The principal technique involves running and comparison of 2D polyacrylamide gels, followed typically by mass spectrometry for identification of the protein spots cut from the gels. The comparative nature of the technique allows identification of protein expression changes across different samples.

· Highly sensitive detection of chemiluminescence and western blots.

· Protein-fluorescence detection on membranes and in SDS-PAGE gels, fluorescence-multiplex western blotting, 1D and 2D gel analyses of nucleic acids and proteins.

· Molecular weight analyses and quantification.

· Gel documentation and nucleic acid fluorescence detection of agarose gels with fluorophores.

· Detection and documentation of protein gels stained with Coomassie Blue, Poisson and silver staining.

· Imaging, documentation and quantitative analysis of dot blots and microtitration plates.

· Colony Counting.