Optima LE-80K
This versatile model is compatible with a wide range of superior rotors and tubes. Instrument classification is R (uses all Beckman Coulter preparative rotors except Types 35 and 42.1 rotors with serial numbers 1299 or lower); not equipped for zonal or continuous-flow operation.
Features:
|
Max Speed: |
80,000 rpm |
|
g Force: |
694,000 x g |
|
Set Speed: |
1,000 to 80,000 rpm in 100-rpm increments |
|
Set Temperature: |
0 to 40° C in 1° C increments |
Applications:
- Separation of proteins by ammonium sulfate precipitation and sucrose/glycerol gradients
- Clarification of lysates and homogenates
- Fractionation of lipoproteins by differential floatation and density gradients
- Isolation of subcellular fractions (microsomes, mitochondria, nuclei, membranes, ribosomes) by pelleting
- Isolation of subcellular fractions (chromatin, mitochondria, ribosomes, microsomes, membranes) by sucrose gradients
- DNA size fractionation in gradients and plasmid purification in CsCl
- Pelleting in CsCl and CsTFA density gradients of RNA
- Isolation of viruses by pelleting and density gradients
Available rotors
Published procedures involving a centrifugation step usually cite the required speed in g-units (i.e. RCF or Relative Centrifugal Force). The centrifuge speed however, is set in rpm (Revolutions Per Minute). In order to convert g-values to rpm, for one of the rotors available in the lab, use the following link - http://www.sciencegateway.org/tools/rotor.htm, http://www.geneinfinity.org/sp/sp_rotor.html, or table:
|
Rotor |
max RCF |
Max speed |
Number of tubes X Nominal Capacity |
Angel description |
|
70.1 Ti |
450,000 |
70,000 |
12 x 13.5 mL |
Fixed angle 30o angle |
|
70 Ti |
504,000 |
70,000 |
8 X 39 mL |
Fixed angle 30o angle |
|
45 Ti |
235,000 |
45,000 |
6 X 94 mL |
Fixed angle 30o angle |
|
SW 55 Ti |
368,000 |
55,000 |
6 x 5 mL |
Swinging Bucket 90o angle |
|
SW 41 Ti |
288,000 |
41,000 |
6 x 13.2 mL |
Swinging Bucket 90o angle |
|
SW 28.1 |
150,000 |
28,000 |
6 x 17 mL |
Swinging Bucket 90o angle |
|
SW 28 |
141,000 |
28,000 |
6 x 38.5 mL |
Swinging Bucket 90o angle |
|
SW 32 Ti |
175000 |
32000 |
6 x 38.5 mL |
Swinging Bucket 90o angle |
|
SW 32.1 Ti |
187000 |
32000 |
6 x 17 mL |
Swinging Bucket 90o angle |
