Incucyte

The Incucyte System is a high throughput, continuous live cell imager.

It is a flexible assay platform that sits inside a standard tissue culture incubator and automatically acquires and analyzes phase and fluorescent images for extended period of time. The systems are used for real-time cell monitoring and surveillance, cell health and viability, migration and invasion, plus a wide range of phenotypic cell-based assays.

We have the Incucyte sx5, sx3 and sx1 systems

Hardware

This is a proprietary system with three objectives:

Objective Magnification/NA Pixel size

Nikon Plan Apo λ x4/0.20 2.82 μm

Nikon Plan Fluor x10/0.30 1.24 μm

Nikon S Plan Fluor x20/0.45 0.62 μm

All objectives have long working distance, many plastic off-the-shelf vessels can be used.

Channel Excitation Emission

Green

Orange

NIR – only in

SX5 Green Orange NIR 453-485 nm 546-568 nm 648-674 nm 494-533 nm 576-639 nm 685-756 nm

Green

Red Green Red 441-481 nm 567-607 nm 503-544 nm 622-704 nm

Metabolism -

only in SX5 ATP 473-498 nm 524-550 nm 565-591 nm Orange NIR 524-550 nm 648-674 nm 565-591 nm 685-756 nm

.

Applications

Cell Counting and Proliferation Viability Apoptosis Cytotoxicity Tumor Spheroid Cell Cycle ATP Metabolism Mitochondrial Membrane Potential Immune Cell Killing Antibody Internalization Phagocytosis NETosis Neuronal Activity Angiogenesis Live-Cell Immunocytochemistry Scratch Wound Migration Invasion Chemotaxis Immune Cell Activation

Manufacture site:

https://www.sartorius.com/en/products/live-cell-imaging-analysis