Exome sequencing

Overview

Exome sequencing is an application used to sequence all the protein-coding genes in a genome (exome), generally used to reveal variants across all coding regions. This application can be used to identify rare and common genetic variants that play a role in human diseases.

Library preparation

Our lab provides library preparation on an initial sample. For the process of Exome Sequencing, libraries are prepared from genomic DNA and then exonic regions are pulled down using specifically designed probs.

Sample requirements

Library Preparation Type	Input Material	Min. Amount	Volume	Dilution Buffer
Exome Sequencing	gDNA	100 ng	< 10 ul	H2O, or 10 mM Tris-Cl, pH 8.5

Sample quality requirements:

Please note that the user is responsible for the quality of each sample. Genomic DNA should be intact. DNA integrity must be confirmed using the Nanodrop, Bioanalyzer, TapeStation, or a similar instrument.

Sequencing

At this time, TAU's Genomics Analysis Lab does not perform sequencing in-house. We prepare and submit libraries to outisde laboratories, such as the Technion Genome Center and G-INCPM, where the sequencing is performed.

Costs

Please contact Dr. Orly Yaron in the Functional Genomics Lab at 03 640 5251 or orlyyaron@post.tau.ac.il for pricing information.

Analysis options

The preliminary analysis from raw datafiles is performed on various platforms such as the Partek Genomics Suite. Further analysis of differentially expressed gene-lists function and pathways is performed using various updated web-based tools. The analysis is generally done on a collaborative basis. For more details, please contact Dr. Metsada Pasmanik-Chor: metsada@post.tau.ac.il , 03 640 6992.