Resequencing analysis is used to reveal genome-wide variations of sequenced data through comparison to a known reference genome. The variants are genotyped, annotated, and compared between the samples.
To prepare for sequencing, our lab provides library preparation on an initial sample of DNA.
|Library Preparation Type||Input Material||Min. Amount||Volume||Dilution Buffer|
||gDNA||500 ng||< 50 ul||H2O, or 10 mM Tris-Cl, pH 8.5|
||gDNA||10 ng||< 10 ul||H2O, or 10 mM Tris-Cl, pH 8.5|
||gDNA||100 ng||< 10 ul||H2O, or 10 mM Tris-Cl, pH 8.5|
||ChIP DNA||20 ng||< 50 ul||H2O, or 10 mM Tris-Cl, pH 8.5|
||Total RNA||1 ug||< 50 ul||H2O|
||Total RNA||1 ug||< 5 ul||H2O|
*Amplicons will be accepted only following DNA purification.
Sample quality requirements:
Please note that the user is responsible for the quality of each sample. Genomic DNA should be intact. Please contact the Lab to coordinate suitable sample prep if your DNA sample is degraded. RNA integrity must be confirmed using the Agilent Bioanalyzer, TapeStation, or a similar instrument, or by running the sample on an agarose gel.
- Sample purity:
OD260/280 = 1.8-2.2
OD260/230 ≥ 2.0
The preliminary analysis from raw datafiles is performed on various platforms such as the Partek Genomics Suite. Further analysis of differentially expressed gene-lists function and pathways is performed using various updated web-based tools. The analysis is generally done on a collaborative basis. For more details, please contact Dr. Metsada Pasmanik-Chor: email@example.com , 03 640 6992.